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用来鉴别与多肽功能、稳定性和构象密切相关的特定的氨基酸位点。丙氨酸（Ala）是最小的手性氨基酸，通过将Ala系统性地、依次地替换序列中每一个氨基酸，来分辨出某一特定氨基酸对整个蛋白生物活性的影响。

应用领域

• 鉴定表位关键基团
• 评估酶的功能活性
• 研究蛋白的结合位点

Overlapping Peptide   Alanine Scanning       Truncation Library   Positional Scanning       Random Library   Scrambled Library       T Cell Truncated Library

参考文献

• Weiss GA, Watanabe CK, Zhong A, Goddard A, and Sidhu SS. Rapid mapping of protein functional epitopes by combinatorial alanine scanning. Proc. Natl. Acad. Sci. U S A. Aug 2000 1; 97(16): 8950-4.
• Richardson PL. The determination and use of optimized protease substrates in drug discovery and development. Curr. Pharm. Des. 2002; 8(28): 2559-81.
• Morrison KL, and Weiss GA. Combinatorial alanine-scanning. Curr. Opin. Chem. Biol. Jun 2001; 5(3): 302-7.
• Levine KB, Hamill S, Cloherty EK, and Carruthers A. Alanine scanning mutagenesis of the human erythrocyte glucose transporter putative ATP binding domain. Blood Cells Mol. Dis. Jan-Feb 2001; 27(1): 139-42.
• Sidhu SS, and Kossiakoff AA. Exploring and designing protein function with restricted diversity. Curr. Opin. Chem. Biol. Jun 2007; 11(3): 347-54.