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Molecular Biology Reagents> | … Samples of the proteins were run on 8%–12% SDS-PAGE gels for immunoblotting. The primary antibodies used were SREBF1 (1:1000, Proteintech, USA), ABCA1 (1:1000, Abcam, UK), and β-actin (1:5000, Genscript, China) … | Get A Quote |
Lipid metabolic disorders have become a major global public health concern. Fatty liver and dyslipidemia are major manifestations of these disorders. Recently, MicroRNA-33 (miR-33), a post-transcriptional regulator of genes involved in cholesterol efflux and fatty acid oxidation, has been considered as a good therapeutic target for these disorders. However, the traditional methods of gene therapy impede their further clinical transformation into a mature treatment system. To counter this problem, in this study we used mesoporous silica nanoparticles (MSNs) as nanocarriers to deliver miR-33 antagomirs developing nanocomposites miR-MSNs. We observed that the hepatocellular uptake of miR-33 antagomirs increased by... More