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Reversibility of hAT-MSCs phenotypic and metabolic changes after exposure to and withdrawal from HCC-conditioned medium through regulation of the ROS/MAPK/HIF-1α signaling pathway

Stem Cell Res Ther. 2020-11; 
Chenyang Wang, Jie Hu, Zheng Chen, Yifan Wang, Sinan Lu, Yuan Zhang, Yufeng Li, Yucheng Xiang, Yutian Ji, Cheng Zeng, Yuan Ding, Weilin Wang
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Molecular Biology Reagents … USA). Next, proteins were separated by 4%–12% SurePAGE Bis-Tris gels (Genscript, Nanjing, China) at consistent 120 V for 60 mins. Then, the proteins were transferred to PVDF membranes at consistent 350 mA for 60 mins … Get A Quote

摘要

background: Mesenchymal stem cells (MSCs) play an important role in tumor progression; concomitantly, MSCs also undergo profound changes in the tumor microenvironment (TME). These changes can directly impact the application and efficacy of MSC-based anti-tumor therapy. However, few studies have focused on the regulation of MSC fate in TME, which will limit the progress of MSC-based anti-tumor therapy. Herein, we investigated the effects of conditioned medium from human hepatocellular carcinoma cells (HCC-CM) on the phenotype and glucose metabolism of human adipose tissue-derived MSCs (hAT-MSCs). methods: The passage 2 (P2) to passage 3 (P3) hAT-MSCs were exposed to conditioned medium from Hep3B, Huh7 and HCCLM3... More

关键词

Cell phenotype, Conditioned medium, Glucose metabolism, Hepatocellular carcinoma, Human adipose tissue-derived mesenchymal stem cells