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Identification of RUNX1T1 as a potential epigenetic modifier in small-cell lung cancer

Mol Oncol. 2020; 
Tian He, Gary Wildey, Karen McColl, Alyssa Savadelis, Kyle Spainhower, Cassidy McColl, Adam Kresak, Aik Choon Tan, Michael Yang, Ata Abbas, Afshin Dowlati
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Molecular Biology Reagents … RUNX1T1 guide RNA (AAGAGTTCGCACCCTCGTTC) on a pLentiCRISPRv2 vector was purchased from GenScript (Piscataway, NJ, USA) and delivered into cells using lenti-viral transduction, as described above. Cells were selected under puromycin at 0.5µg/ml for about … Get A Quote

摘要

Small-cell lung cancer (SCLC) can be subgrouped into common 'pure' and rare 'combined' SCLC (c-SCLC). c-SCLC features a mixed tumor histology of both SCLC and non-small-cell lung cancer (NSCLC). We performed targeted exome sequencing on 90 patients with SCLC, including two with c-SCLC, and discovered RUNX1T1 amplification specific to small cell tumors of both patients with c-SCLC, but in only 2 of 88 'pure' SCLC patients. RUNX1T1 was first identified in the fusion transcript AML1/ETO, which occurs in 12%-15% of acute myelogenous leukemia (AML). We further show higher expression of RUNX1T1 in the SCLC component of another c-SCLC tumor by in situ hybridization. RUNX1T1 expression was enriched in SCLC compared wit... More

关键词

E2F pathway, RUNX1T1, epigenetics, exome sequencing, small-cell lung cancer