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Thermobifida fusca cellulases exhibit increased endo-exo synergistic activity, but lower exocellulase activity, on cellulose-III

ACS Sustainable Chem. Eng. 2020; 
Yuxin Liu, Bhargava Nemmaru, and Shishir P.S. Chundawat
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Molecular Biology Reagents T. fusca β-glucosidase gene with an N-terminus 8X His was ordered from Genscript (Piscataway, NJ) and cloned directly into a standard pET26b(+) vector using SLIC. Get A Quote

摘要

Cellulose recalcitrance toward saccharification is a barrier for low-cost biofuels production. Ammonia-based pretreatments can alter the native cellulose-I allomorphic state to form an unnatural cellulose-III allomorph that is less recalcitrant toward enzymatic hydrolysis. Here, we characterize the hydrolytic activity of a thermophilic cellulolytic microbe, Thermobifida fusca, derived cellulase on cellulose-III. Up to 2-fold improved activity was observed for homologously expressed T. fusca cellulase enzymes on cellulose-III. Surprisingly, T. fusca exocellulases like Cel6B alone had lower activity on cellulose-III. We hypothesized that increased activity of T. fuscacellulases on cellulose-III arises mostl... More

关键词

Cellulosic Biofuels, Thermobifida fusca, Cellulases, Ammonia Pretreatment, Cellulose Allomorphs, Endo-Exo Cellulase Synergy