Products/Services Used | Details | Operation |
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Molecular Biology Reagents> | pLenti-PGK-Degron-GFP-Blast, pLenti-PGK-Degron-GFP-Klhl13-Blast, pLenti-PGK-GFP-Blast, and pLenti-PGK-GFP-Kelch, which were used to identify Klhl13 interaction partners were generated and cloned into the pLenti-PGK-GFP-Blast lentiviral plasmid (Addgene 19069, (Campeau et al., 2009)) by GenScript (Suppl. Table S4). | Get A Quote |
X-chromosomal genes contribute to sex differences, in particular during early development, when both X chromosomes are active in females. Here, double X-dosage shifts female pluripotent cells towards the naive stem cell state by increasing pluripotency factor expression, inhibiting the differentiation-promoting MAP kinase (MAPK) signalling pathway and delaying differentiation. To identify the genetic basis of these sex differences, we have performed a series of CRISPR knockout screens in murine embryonic stem cells to comprehensively identify X-linked genes that cause the female pluripotency phenotype. We found multiple genes that act in concert, among which Klhl13 plays a central role. We show that this E3 ubi... More