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Molecular Biology Reagents> | The strains were transformed by a lithium-acetate-heat shock method with a plasmid containing the Hc-LPT1 gene (Uniprot ID C0NZS2) that was codon optimized, synthesized and clone into the Galinducible plasmid pESC-URA by GenScript (Piscataway, NJ). | Get A Quote |
Lipids play a fundamental role in fungal cell biology, being components of the cell membrane as well as targets of antifungal drugs. A deeper knowledge of lipid metabolism is key for developing new drugs and a better understanding of fungal pathogenesis. Here we built a comprehensive map of the Histoplasma capsulatum lipid metabolic pathway by integrating proteomic and lipidomic analyses. The map led to the identification of both the fatty acid desaturation and the sphingolipid biosynthesis pathways as targets for drug development. We also found that H. capsulatumproduces analogs of platelet-activating factor, a potent regulator of the human immune response. The H. capsulatum platelet-activating factor ana... More