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Determination of Parvovirus Retention Profiles in Virus Filter Membranes Using Laser Scanning Microscopy

Journal of Membrane Science. 2020; 
Remo Leisi a, *, Jan Bieri a, Nathan J. Roth b, Carlos Ros a
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Molecular Biology Reagents A plasmid encoding the bacteriophage MS2 coat protein for the expression of recombinant MS2 virus-like particles (VLPs) was purchased from GenScript (Nanjing, China). Get A Quote

摘要

Virus filtration is a highly effective method in the downstream processing of biotherapeutic products to provide effective removal of potential infectious agents based on a size exclusion mechanism. The direct visualization of viruses retained inside the filter membrane represents a valuable tool to get a deeper understanding of the filtration process and to explain observations of virus breakthrough under particular operating conditions. Parvoviruses, which are used as worst-case models in validation studies, were purified and labeled with fluorescent dyes to detect their retention pattern inside the filter membrane using laser scanning microscopy. Critical factors influencing the reproducibility and accuracy ... More

关键词

Virus filtration Nanofiltration Filter membrane Parvovirus retention profile Laser scanning microscopy