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A Pathogen-Responsive Gene Cluster for Highly Modified Fatty Acids in Tomato.

Cell. 2020; 
Jeon JE, Kim JG, Fischer CR, Mehta N, Dufour-Schroif C, Wemmer K, Mudgett MB, Sattely E.
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摘要

In response to biotic stress, plants produce suites of highly modified fatty acids that bear unusual chemical functionalities. Despite their chemical complexity and proposed roles in pathogen defense, little is known about the biosynthesis of decorated fatty acids in plants. Falcarindiol is a prototypical acetylenic lipid present in carrot, tomato, and celery that inhibits growth of fungi and human cancer cell lines. Using a combination of untargeted metabolomics and RNA sequencing, we discovered a biosynthetic gene cluster in tomato (Solanum lycopersicum) required for falcarindiol production. By reconstituting initial biosynthetic steps in a heterologous host and generating transgenic pathway mutants in tomato... More

关键词

acetylenase; acetylenic lipids; antifungal oxylipin; falcarindiol; plant lipid gene cluster; plant natural product biosynthesis; transcriptomics; untargeted metabolomics