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An enhanced sensitivity methyl H triple-quantum pulse scheme for measuring diffusion constants of macromolecules.

J. Biomol. NMR. 2017; 
Huang Rui,Brady Jacob P,Sekhar Ashok,Yuwen Tairan,Kay Lew
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Custom Vector Construction synthesized (Genscript) and sub-cloned into pET-SUMO vectors. Briefly, N-terminal His-SUMO constructs were Get A Quote

摘要

We present a pulse scheme that exploits methyl H triple-quantum (TQ) coherences for the measurement of diffusion rates of slowly diffusing molecules in solution. It is based on the well-known stimulated echo experiment, with encoding and decoding of TQ coherences. The size of quantifiable diffusion coefficients is thus lowered by an order of magnitude with respect to single-quantum (SQ) approaches. Notably, the sensitivity of the scheme is high, approximately ? that of the corresponding single quantum experiment, neglecting relaxation losses, and on the order of a factor of 4 more sensitive than a previously published sequence for AX spin systems (Zheng et al. in JMR 198:271-274, 2009) for molecules... More

关键词

Ddx4,Diffusion,Methyl groups,NMR,Triple-qua