| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | Full-length cDNAs for all other predicted peptide receptors in Ae. aegypti L3 and L5 genome annotations were cloned from cDNA isolated from 10 whole mosquitoes (Liverpool strain) or synthesized by GenScript and subcloned with XhoI-NotI into the pME18s vector for expression in mammalian cells (Data S1). | Get A Quote |
Female Aedes aegypti mosquitoes bite humans to obtain blood to develop their eggs. Remarkably， their strong attraction to humans is suppressed for days after the blood meal by an unknown mechanism. We investigated a role for neuropeptide Y (NPY)-related signaling in long-term behavioral suppression and discovered that drugs targeting human NPY receptors modulate mosquito host-seeking. In a screen of all 49 predicted Ae. aegypti peptide receptors， we identified NPY-like receptor 7 (NPYLR7) as the sole target of these drugs. To obtain small-molecule agonists selective for NPYLR7， we performed a high-throughput cell-based assay of 265，211 compounds and isolated six highly selective NPYLR7 agonists that inh... More
