Protein inhibitor of activated STAT (PIAS) proteins are activation-suppressing proteins for signal transducer and activator of transcription (STAT)， which involves gene transcriptional regulation. The inhibitory mechanism of PIAS proteins in the Janus kinase (JAK)/STAT signaling pathway has been well studied in mammals and . However， the roles of PIAS in crustaceans are unclear. In the present study， we identified PIAS in kuruma shrimp and found that its relative expression could be induced by stimulation. To explore the function of PIAS in shrimp infected with ， we performed an RNA interference assay. After knockdown of expression in shrimp subjected to infection， bacterial clearance was enhanced... More
Protein inhibitor of activated STAT (PIAS) proteins are activation-suppressing proteins for signal transducer and activator of transcription (STAT)， which involves gene transcriptional regulation. The inhibitory mechanism of PIAS proteins in the Janus kinase (JAK)/STAT signaling pathway has been well studied in mammals and . However， the roles of PIAS in crustaceans are unclear. In the present study， we identified PIAS in kuruma shrimp and found that its relative expression could be induced by stimulation. To explore the function of PIAS in shrimp infected with ， we performed an RNA interference assay. After knockdown of expression in shrimp subjected to infection， bacterial clearance was enhanced and the survival rate increased compared with those in the control shrimp ( injection). Simultaneously， the expression levels of antimicrobial peptides (AMPs)， including anti-lipopolysaccharide factor (ALF) A1， C1， C2， and CruI-1， increased. Further study revealed that knockdown of also enhanced STAT phosphorylation and translocation. Pulldown assay indicated that PIAS interacts with activated STAT in shrimp. In conclusion， PIAS negatively regulates JAK/STAT signaling by inhibiting the phosphorylation and translocation of STAT through the interaction between PIAS and STAT， which leads to the reduction of AMP expression in shrimp. Our results revealed a new mechanism of PIAS-mediated gene regulation of the STAT signal pathway.
