至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage.

Braz. J. Med. Biol. Res.. 2017; 
ZhangC,YuanJ,HuH,ChenW,LiuM,ZhangJ,SunS,
Products/Services Used Details Operation
Custom Vector Construction The siRNA for CHCHD4P4 (5′-CAUGGAUUGAUACUACCAATT-3′) and a negative-control siRNA (5′-UUCUCCGAACGUGUCACGUdTdT-3′) were purchased from GenePharma (China). The CHCHD4P4 gene was cloned into the expression vector pcDNA3.1 (GenScript, China). Get A Quote

摘要

Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs we... More

关键词