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Custom Vector Construction> | The siRNA for CHCHD4P4 (5′-CAUGGAUUGAUACUACCAATT-3′) and a negative-control siRNA (5′-UUCUCCGAACGUGUCACGUdTdT-3′) were purchased from GenePharma (China). The CHCHD4P4 gene was cloned into the expression vector pcDNA3.1 (GenScript, China). | Get A Quote |
Kidney stone disease is a major cause of chronic renal insufficiency. The role of long non-coding RNAs (lncRNAs) in calcium oxalate-induced kidney damage is unclear. Therefore, we aimed to explore the roles of lncRNAs in glyoxylate-exposed and healthy mouse kidneys using microarray technology and bioinformatics analyses. A total 376 mouse lncRNAs were differentially expressed between the two groups. Using BLAST, 15 lncRNA homologs, including AU015836 and CHCHD4P4, were identified in mice and humans. The AU015836 expression in mice exposed to glyoxylate and the CHCHD4P4 expression in human proximal tubular epithelial (HK-2) cells exposed to calcium oxalate monohydrate were analyzed, and both lncRNAs we... More