| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | The CDS sequence (1596 bp) of the SHR gene (At4g37650) was cloned in the pCAMBIA 5300 vector (CAMBIA, Canberra, Australia) by the Genscript company (Piscataway, NY state, US) using XmaI/KpnI restriction sites, and the of the OsSHR2 CDS sequence (OsO3g31880) (1812 pb) was cloned in the lab using XmaI restriction sites. | Get A Quote |
The number of root cortex cell layers varies among plants， and many species have several cortical cell layers. We recently demonstrated that the two rice orthologs of the Arabidopsis SHR gene， OsSHR1 and OsSHR2， could complement the A. thaliana shr mutant. Moreover， OsSHR1 and OsSHR2 expression in A. thaliana roots induced the formation of extra root cortical cell layers. In this article， we demonstrate that the overexpression of AtSHR and OsSHR2 in rice roots leads to plants with wide and short roots that contain a high number of extra cortical cell layers. We hypothesize that SHR genes share a conserved function in the control of cortical cell layer division and the number of ground tissue cell laye... More
