Reverse genetics approaches have become powerful tools to dissect the biology of malaria parasites. In a previous study， development of an in vitro drug selection method for generating transgenic parasite of Plasmodium berghei was reported. Using this method， two novel and independent selection markers using the P. berghei heat shock protein 70 promoter was previously established. While the approach permits the easy and flexible genetic manipulation of P. berghei， shortcomings include a low variety in promoter options to drive marker gene expression and increased complexity of the selection procedure. In this study， addressing these issues was attempted.
Reverse genetics approaches have become powerful tools to dissect the biology of malaria parasites. In a previous study， development of an in vitro drug selection method for generating transgenic parasite of Plasmodium berghei was reported. Using this method， two novel and independent selection markers using the P. berghei heat shock protein 70 promoter was previously established. While the approach permits the easy and flexible genetic manipulation of P. berghei， shortcomings include a low variety in promoter options to drive marker gene expression and increased complexity of the selection procedure. In this study， addressing these issues was attempted.
