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Cloning， expression and characterization of a cold-adapted endo-1， 4--glucanase from A1， a symbiotic bacterium of .

PeerJ. 2016; 
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DNA Sequencing … step of 6 min at 95 °C, a second step of 35 cycles including 35 s at 95 °C, 1 min at 60 °C and 1 min at 72 °C, and a final step of 8 min at 72 °C The DNA product was cloned into the pMD20-T vector using standard procedures and sequenced by Genscript Corporation (China) … Get A Quote

摘要

Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study， A1 which was isolated from a wood-inhabiting termite could secrete a cold-adapted EglC. However， its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.8 U/ml). The objective of the present study was to clone and express the gene in to improve production level and determine the enzymatic properties of the recombinant enzyme.

关键词

Citrobacter farmeri Cold-adapted Endoglucanase Escherichia coli Expression Properties
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