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Cloning， expression and characterization of a cold-adapted endo-1， 4--glucanase from A1， a symbiotic bacterium of .

PeerJ. 2016;

Products/Services Used	Details	Operation
DNA Sequencing	… step of 6 min at 95 °C, a second step of 35 cycles including 35 s at 95 °C, 1 min at 60 °C and 1 min at 72 °C, and a final step of 8 min at 72 °C The DNA product was cloned into the pMD20-T vector using standard procedures and sequenced by Genscript Corporation (China) …	Get A Quote

摘要

Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study， A1 which was isolated from a wood-inhabiting termite could secrete a cold-adapted EglC. However， its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.8 U/ml). The objective of the present study was to clone and express the gene in to improve production level and determine the enzymatic properties of the recombinant enzyme.