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Label-free electrochemical detection of botulinum neurotoxin type E based on its enzymatic activity using interdigitated electrodes

Appl. Phys. Lett.. 2016-09; 
Sang Hwa Hyun1, Dae Keun Park1, Aeyeon Kang1, Soohyun Kim1, Daehee Kim1, Yu Mi Shin2, Ji-Joon Song2, and Wan Soo Yun
Products/Services Used Details Operation
Custom Vector Construction BoNT/E LC was purchased from Genscript (USA) and cloned to modify pET28a vector. Get A Quote

摘要

We report a simple label-free electrochemical method of detecting low concentrations of botulinum neurotoxin type E light chain (BoNT/E LC) based on its peptide cleavage activity. Dual-mode cyclic voltammetry was employed to observe changes in the redox signal of ferri-/ferro-cyanide on interdigitated microelectrodes, whose surfaces were covered by peptides designed from synaptosomal-associated protein 25 to be cleaved by BoNT/E LC. With the introduction of BoNT/E LC, the redox signal showed a time-dependent increase due to cleavage of the immobilized peptide molecules. In addition to the increased redox signal intensity, its time-dependence can be considered as a strong evidence of BoNT/E sensing, since the ti... More

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