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Synonymous mutation gene design to overexpress ACCase in creeping bentgrass to obtain resistance to ACCase-inhibiting herbicides.

Transgenic Res.. 2016-09; 
HeckartDouglas L,SchwartzBrian M,RaymerPaul L,ParrottWay
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Custom Vector Construction … ACCase to ensure 100 % identity. The modified gene was synthesized without any further modification by Genscript (Piscataway, NJ) and deposited in GenBank (accession KP859588). Vector construction. Four genes were used in … Get A Quote

摘要

Overexpression of a native gene can cause expression of both introduced and native genes to be silenced by posttranscriptional gene silencing (PTGS) mechanisms. PTGS mechanisms rely on sequence identity between the transgene and native genes; therefore, designing genes with mutations that do not cause amino acid changes, known as synonymous mutations, may avoid PTGS. For proof of concept, the sequence of acetyl-coA carboxylase (ACCase) from creeping bentgrass (Agrostis stolonifera L.) was altered with synonymous mutations. A native bentgrass ACCase was cloned and used as a template for the modified gene. Wild-type (WT) and modified genes were further modified with a non-synonymous mutation, coding fo... More

关键词

Acetyl coenzyme A carboxylase (ACCase),Aryloxyphenoxypropionate (APP),Cyclohexandione (CHD),Herbicide-resistance,Posttranscriptional gene silencing,Sethoxydim resistant,Synonymous mutation,Synthetic gene de