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Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1.

J. Virol.. 2015-05; 
NakaharaTomomi,TanakaKatsuyuki,OhnoShin-ichi,EgawaNagayasu,YugawaTakashi,KiyonoT
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Custom Vector Construction Lentivirus vectors, CSII-CMV-tetON-ADV and CSII-TRE-Tight-HA16E1, were described previously (38). The codon-optimized HPV16 E2 cDNA (GenScript, Piscataway, NJ) was used to construct CSII-TRE-Tight-HA16E2, Get A Quote

摘要

NF-κB is a family of transcription factors that regulate gene expression involved in many processes, such as the inflammatory response and cancer progression. Little is known about associations of NF-κB with the human papillomavirus (HPV) life cycle. We have developed a tissue culture system to conditionally induce E1-dependent replication of the human papillomavirus 16 (HPV16) genome in human cervical keratinocytes and found that expression of HPV16 E1, a viral helicase, results in reduction of IκBα and subsequent activation of NF-κB in a manner dependent on helicase activity. Exogenous expression of a degradation-resistant mutant of IκBα, which inhibits the activation of NF-κB, enhanced E1-d... More

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