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Inhibiting plant microRNA activity: molecular SPONGEs, target MIMICs and STTMs all display variable efficacies against target microRNAs.

Plant Biotechnol. J.. 2015; 
ReichelMarlene,LiYanjiao,LiJunyan,MillarAntho
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Custom Vector Construction All SPs and STTMs sequences were synthesized [GenScript (USA) or IDT (USA)] and cloned into the Gateway donor vector pDONR/Zeo (Invitrogen). Get A Quote

摘要

Elucidation of microRNA (miRNA) function through a loss-of-function approach has proven difficult due to extensive genetic redundancy among most plant and animal miRNA families. Consequently, miRNA decoy technologies such as target MIMICs (MIMs) and short tandem target MIMICs (STTMs) in plants or molecular SPONGEs (SPs) in animals have been developed to generate loss-of-function phenotypes by perturbing endogenous miRNA activity. To test whether SPs can inhibit plant miRNA activity, synthetic SP transgenes containing multiple miRNA binding sites targeting different Arabidopsis miRNA families were generated. Additionally, their silencing efficacies were compared to the corresponding MIM and STTM transgenes... More

关键词

MIMICs,STTMs,microRNA,molecular SPONGE,ΔG free en