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Role of the Pre-neck Appendage Protein (Dpo7) from Phage vB_SepiS-phiIPLA7 as an Anti-biofilm Agent in Staphylococcal Species.

Front Microbiol. 2015; 
GutiérrezDiana,BriersYves,Rodríguez-RubioLorena,MartínezBeatriz,RodríguezAna,LavigneRob,GarcíaP
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Custom Vector Construction … Additionally, NdeI and XhoI restriction sites were added at the 5′ and 3′ ends of the sequence, respectively The optimized sequence was synthesized and cloned into pUC57 vector by GenScript (Township, NJ, USA) Afterward … Get A Quote

摘要

Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 μM of Dpo7 in all polysaccharide producer strains but Dpo7 faile... More

关键词

S. aureus,S. epidermidis,biofilm,biofilm matrix,exopolysaccharide depolyme