Products/Services Used | Details | Operation |
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Custom Vector Construction> | … Preparation of rSPA antigen A 245 bp fragment of the rSPA gene (verified via the National Center for Biotechnology Information website) was optimized based on E coli codon usage, synthesized by GenScript, and subcloned into the pET-44a expression vector … | Get A Quote |
In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and moderate/poor differentiation. We show that miR-145 targets the DNA damage repair-associated gene Helicase-like transcription factor (HLTF), which is involved in radio-resistance. Moreover, miR-145 over-expression in cervical cancer cells enhances radiosensitivity in vitro and in vivo. These results indicate that targeting miR-145 may be a novel radiosensitizing strategy for cervical cance... More