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The miR-125a/HK2 axis regulates cancer cell energy metabolism reprogramming in hepatocellular carcinoma.

Sci Rep. 2017; 
JinFangfang,WangYanbo,ZhuYanan,LiShan,LiuYing,ChenCheng,WangXiaohua,ZenKe,LiL
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Custom Vector Construction For the HK2 overexpression assay, a pcDNA3.1 vector was designed to specifically express the open reading frame (ORF) of human HK2 containing full-length 3′-UTR and purchased from GenScript (Nanjing, China).For the luciferase reporter assay, p-MIR-REPORT plasmids were designed to contain the 3′-untranslated region (UTR) of human HK2 and purchased from GenScript (Nanjing, China). Get A Quote

摘要

The Warburg effect is a metabolic hallmark of cancer. Tumor cells rapidly adjust their energy source to glycolysis in order to efficiently proliferate in a hypoxic environment, but the mechanism underlying this switch remains incompletely understood. Here, we show that hypoxia potently induces the down-regulation of miR-125a expression in hepatocellular carcinoma (HCC) cells and tumors. Furthermore, we demonstrate that miR-125a could decrease the production of lactate, the uptake of glucose, and the levels of ATP and reactive oxygen species (ROS) in HCC cells. We investigated the molecular mechanism through which miR-125a inhibits HCC glycolysis and identified hexokinase II (HK2) as a direct target ge... More

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