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A recombinant Fasciola gigantica 14-3-3 epsilon protein (rFg14-3-3e) modulates various functions of goat peripheral blood mononuclear cells.

Parasit Vectors. 2018-01;

TianAi-Ling，LuMingMin，Calderón-MantillaGuillermo，PetsalakiEvangelia，DottoriniTania，TianXiaoWei，WangYuJian，HuangSi-Yang，HouJun-Ling，LiXiangRui，ElsheikhaHany M，ZhuXing-

Products/Services Used	Details	Operation
DNA Sequencing	… Several positive clones were selected and sequenced by GenScript (Nanjing, Jiangsu, China) to confirm the correct insertion/orientation of Fg14-3-3e gene in the vector. Characterization of the Fg14-3-3e gene sequence. We …	Get A Quote

摘要

The molecular structure of Fasciola gigantica 14-3-3 protein has been characterized. However， the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e)， and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs).