| Species |
Mouse |
| Protein Construction |
| hFc |
OX40 Ligand/TNFSF4 (Ser51-Leu198)_x000D_
Accession # P43488 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per ug by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized OX40 Ligand/TNFSF4 hFc Chimera, Mouse at 5μg/ml (100μl/well) on the plate can bind Mouse OX40, His Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
42.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 52-56 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. |
| Synonyms |
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L; TXGP1; Glycoprotein Gp34 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
