目录产品 » TDGF1/Cripto, His, Human
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TDGF1/Cripto, His, Human

TDGF1 (CRIPTO) is a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic (EGF/CFC) gene family and an obligate co-receptor involved in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. Cripto-1 is enriched in a subpopulation of embryonal, melanoma, prostate, and pancreatic cancer cells that possess stem-like characteristics. Therefore, Cripto-1 may play a role during developmental EMT, and it may also be involved in the reprogramming of differentiated tumor cells into cancer stem cells through the induction of an EMT program.
￥3000
Z05863-100

产品简介
Species Human
Protein Construction
TDGF1/Cripto (Leu31-Thr172)
Accession # P13385		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Loaded Human ACVR1B, hFc Tag on ProA­Biosensor can bind TDGF1/Cripto, His, Human in BLI assay (Gator® Prime). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 16.92 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
TDGF1/Cripto, His, Human

TDGF1/Cripto, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

TDGF1/Cripto, His, Human

TDGF1/Cripto, His, Human on CM5 Chip via Protein A can bind Human TDGF1, His Tag with an affinity constant of 0.16 μM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background TDGF1 (CRIPTO) is a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic (EGF/CFC) gene family and an obligate co-receptor involved in NODAL signaling, a developmental program implicated in midline, forebrain, and left-right axis development in model organisms. Cripto-1 is enriched in a subpopulation of embryonal, melanoma, prostate, and pancreatic cancer cells that possess stem-like characteristics. Therefore, Cripto-1 may play a role during developmental EMT, and it may also be involved in the reprogramming of differentiated tumor cells into cancer stem cells through the induction of an EMT program.
Synonyms TDGF1; Cripto; CRGF; CRIPTO; CR
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.