目录产品 » SMPD1, His, Human
Manual
SDS
COAs

SMPD1, His, Human

Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.
￥4100
Z05849-100

产品简介
Species Human
Protein Construction
SMPD1 (Met1-Pro628)_x000D_
Accession # NP_000534.3		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its ability to cleave 2­N­Hexadecanoylamino­4­nitrophenylphosphorylcholine (HNPPC).Test result meets the standard.
Expression System Baculovirus-Insect Cells
Theoretical Molecular Weight 66.53 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result.
Formulation Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).
Concentration Verified by one or more methods from 0.64mg/ml A280/Bioactivity/BCA/Bradford.
Storage & Stability This product remains stable for 6 months at -80°C or below. Avoid repeated freeze-thaw cycles.
返回

技术背景
Target Background Sphingomyelin phosphodiesterase 1 (SMPD1) converts sphingomyelin into ceramide and phosphocholine; hence, loss of SMPD1 function causes abnormal accumulation of sphingomyelin in lysosomes, which results in the lipid-storage disorder Niemann-Pick disease (types A and B). SMPD1 activity is dependent on zinc, which is coordinated at the active site of the enzyme.
Synonyms ASM;
返回

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.