目录产品 » PKC iota, His, Human
Manual
SDS
COAs

PKC iota, His, Human

PKClambda/iota belongs to the third group of the PKC family, atypical PKC (aPKC), together with PKCzeta based on its sequence divergence from conventional and novel PKCs observed not only in the N-terminal regulatory domain but also in the kinase domain. Although one of the most distinct features of aPKC is its single, unrepeated cysteine-rich domain, recent studies have revealed that the N-terminal regulatory domain has additional aPKC-specific structural motifs involved in various protein-protein interactions, which are important for the regulation and the subcellular targeting of aPKC.
￥3300
Z05754-100

产品简介
Species Human
Protein Construction
PKC iota (Met10-Val596)_x000D_
Accession # P41743		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System Baculovirus-Insect Cells
Theoretical Molecular Weight 68.78 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
Formulation Supplied as 0.22 μm filtered solution in 50mM Tris, 100mM NaCl, 0.5mM GSH, 0.5mM PMSF, 10% glycerol (pH 7.4).
Concentration Verified by one or more methods from 0.64mg/ml A280/Bioactivity/BCA/Bradford.
Storage & Stability This product remains stable for 6 months at -80°C or below. Avoid repeated freeze-thaw cycles.
返回

技术背景
Target Background PKClambda/iota belongs to the third group of the PKC family, atypical PKC (aPKC), together with PKCzeta based on its sequence divergence from conventional and novel PKCs observed not only in the N-terminal regulatory domain but also in the kinase domain. Although one of the most distinct features of aPKC is its single, unrepeated cysteine-rich domain, recent studies have revealed that the N-terminal regulatory domain has additional aPKC-specific structural motifs involved in various protein-protein interactions, which are important for the regulation and the subcellular targeting of aPKC.
Synonyms nPKC-iota; PRKCI; DXS1179E;
返回

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.