| Species |
Human |
| Protein Construction |
Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer [Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail]
Accession # P06756-1(ITGAV)&P18084(ITGB5) |
His |
Avi |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer, His & Avi, Human at 2μg/ml (100μl/Well) on the plate can bind Antialpha V Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
114 kDa (ITGAV) & 81.4 kDa (ITGB5) |
| Apparent Molecular Weight |
Due to glycosylation,the protein migrates to 85-140 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection) Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C. |
| Synonyms |
αVβ5; Integrin alpha V beta 5; beta5; alphaV; ITGAV&ITGB5;Integrin alpha V β 5; β5 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
