| Species |
Human |
| Protein Construction |
IL-12 R beta 1/CD212 (Cys24-Glu540)
Accession # P42701-1 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human IL12B, His Tag at 5μg/ml (100μl/well) on the plate can bind IL-12 R beta 1/CD212 hFc Chimera, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
83.85 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 95-115 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human IL-12B, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-12 R beta 1/CD212 hFc Chimera, Human, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA.
IL-12 R beta 1/CD212 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function). |
| Synonyms |
IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454;IL-12R-β-1; IL-12R subunit β-1; IL-12R-β1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
