| ￥5800 | |
| Z04628-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||||
| Endotoxin Level | Less than 1 EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse DR3, His Tag at 1 μg/ml (100 μl/well) on the plate can bind TNFSF15[Biotin], His & Avi, Human. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 23.38 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 27-37 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Mouse DR3, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for TNFSF15[Biotin], His & Avi, Human, His Tag with the EC50 of 45.6 ng/ml determined by ELISA. »
TNFSF15[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Immobilized Human DR3, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for TNFSF15[Biotin], His & Avi, Human , His Tag with the EC50 of 72.2ng/ml determined by ELISA. »
Immobilized TNFSF15[Biotin], His & Avi, Human , His Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. »
| Target Background | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
| Synonyms | TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
