| Species |
Human |
| Protein Construction |
Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer [Phe31-Val992 (ITGAV) acidic tail & Gly27-Asp718 (ITGB3) basic tail]
Accession # P06756-1(ITGAV) & P05106-1 (ITGB3) |
His |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer[Biotin], His & Avi, Human at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind Antialpha V beta3 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
114 kDa (ITGAV) & 81.3 kDa (ITGB3) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 100-160 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. |
| Synonyms |
αVβ3; Integrin alpha V beta 3; beta3; alphaV; ITGAV&ITGB3;Integrin alpha V β 3; β3 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
