| ￥5800 | |
| Z04473-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human GITR, hFc Tag at 2μg/ml (100μl/well) on the plate can bind GITR Ligand/TNFSF18 Trimer [Biotin], His & Flag, Human. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 47.5 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human GITR, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for GITR Ligand/TNFSF18 Trimer [Biotin], His & Flag, Human, His Tag with the EC50 of 11.4 ng/ml determined by ELISA. »
The purity of GITR Ligand/TNFSF18 Trimer [Biotin], His & Flag, Human is greater than 95% as determined by SEC-HPLC. »
GITR Ligand/TNFSF18 Trimer [Biotin], His & Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Glucocorticoid-induced TNFR-related protein (TNFRSF18, GITR, CD357), expressed by T cells, and its ligand (TNFSF18, GITRL), expressed by myeloid populations, provide co-stimulatory signals that boost T cell activity. Due to the important role that GITR plays in regulating immune functions, agonistic stimulation of GITR is a promising therapeutic concept. |
| Synonyms | TNFSF18; AITRL; TL6; GITRL; GITR Ligand |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
