| ￥3300 | |
| Z04273-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized IL-2 R alpha/CD25, His & Avi, Human at 0.5μg/ml (100μl/well) on the plate can bind AntiIL2 R alpha Antibody, hFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 24.6 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μL/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA. »
The purity of IL-2 R alpha/CD25, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
IL-2 R alpha/CD25, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Immobilized IL-2 R alpha/CD25, His & Avi, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 5.3 ng/ml determined by ELISA. »
IL-2 R alpha/CD25, His & Avi, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). »
| Target Background | Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
| Synonyms | CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
