| Species |
Human |
| Protein Construction |
CD3E & CD3G [Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)]
Accession # P07766 (CD3E) & P09693 (CD3G) |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3G hFc Chimera, Human at 1μg/ml (100μl/well) on the plate can bind Anti-CD3E&CD3G Ab.2, mFc Tag . Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
37.8 kDa (CD3E) and 36.5 kDa (CD3G) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from a 0.22 μm filtered solution in PBS，(pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized CD3E&CD3G hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CD3 Antibody, mFc Tag with the EC50 of 29.8 ng/ml determined by ELISA.
The purity of CD3E&CD3G hFc Chimera, Human was greater than 95% as determined by SEC-HPLC.
CD3E&CD3G hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
| Synonyms |
CD3; CD3G; CD3g; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
