目录产品 » MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human
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MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
￥5800
Z03868-100

产品简介
Species Human
Protein Construction
MSLN/Mesothelin (Glu296-Gly580)
Accession # Q13421-2		 hFc
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Immobilized Human CA125, His Tag at 2 μg/ml (100μl/Well) on the plate can bind MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human (Cat.No.: Z03868).
Expression System HEK293
Theoretical Molecular Weight 61.1 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, pH 7.4.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
MSLN/Mesothelin(296-580) HFc Chimera[Biotin], Avi, Human

Immobilized Human CA125, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 39.9 ng/ml determined by ELISA. »

MSLN/Mesothelin(296-580) HFc Chimera[Biotin], Avi, Human

The purity of MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human is greater than 95% as determined by SEC-HPLC. »

MSLN/Mesothelin(296-580) HFc Chimera[Biotin], Avi, Human

MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

MSLN/Mesothelin(296-580) HFc Chimera[Biotin], Avi, Human

Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 18.4 ng/ml determined by ELISA. »

MSLN/Mesothelin(296-580) HFc Chimera[Biotin], Avi, Human

Use MSLN/Mesothelin(296-580) hFc Chimera[Biotin], Avi, Human to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN. »

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技术背景
Target Background Mesothelin, also known as MSLN, is encoded by the MSLN gene. It encodes a precursor protein of 71 kDa that is processed to a 31 kDa shed protein called megakaryocyte potentiating factor (MPF) and a 40 kDa fragment, mesothelin. MSLN is attached to the cell membrane by a glycosyl-phosphatidylinositol (GPI) anchor. It is a differentiation antigen which is highly expressed in several human cancers, including virtually all mesotheliomas and pancreatic adenocarcinomas.
Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.