GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.
|Cat. No.||Product Name
|L00680-12||Protein A Resin FF (1mlx2)||1mlx2||50% slurry|
|L00680-51||Protein A Resin FF (5mlx1)||5mlx1||50% slurry|
|L00680-55||Protein A Resin FF (5mlx5)||5mlx5||50% slurry|
|Ligand||Recombinant Streptococcal protein A expressed in E. coli|
|Number of IgG binding sites per ligand||5|
|M.W. of ligand||Approximately 34 kDa|
|PI of ligand||5.17|
|Degree of substitution||Approximately 5 mg protein A / ml settled resin|
|Static binding capacity||> 40 mg human IgG/ ml settled resin|
|Matrix component||Agarose, 4% highly cross-linked|
|Average particle size||90 μm (45-165 μm)|
|Storage solution||20% ethanol |
|Storage conditions||2-8 °C|
|Shelf life||18 months when stored unopened|
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).