|Description||Broad Multi Color Pre-Stained Protein Standard is a special-designed protein standard. The standard is a mixture of ten highly purified prestained proteins ranging from 5 kDa to 270 kDa, covalently coupled with four different chromophores. The 30 kDa band is red, 50 kDa band is green, 270 kDa band is orange, and other bands are blue. Broad Multi Color Pre-Stained Protein Standard is designed for observing protein separation during SDS-PAGE, verifying western transfer efficiency on membranes, and approximating the size of proteins.|
|Volume||Bands intensity may vary according to gel type, thickness, and staining condition. Please adjust loading volume from 2.5 ul to10 μl.|
|Calculation||The apparent molecular weights of its ten protein bands are 5kD, 15kD, 30kD, 35kD, 50kD, 65kD, 95kD,130kD,175kD, and 270kD.|
|Reconstitution||Thaw this product at room temperature. Do NOT boil protein ladder. Mix gently and thoroughly to ensure that the solution is homogeneous.|
|Storage||-20°C for up to one year or 4°C for up to three months.|
|Note||1. Non-BSA based blocking reagents (such as 5% skimmed milk) are recommended for blocking.
2. For best results, the loading volume of the marker should be optimized for different experiment conditions (from 2.5 µl to 10 µl).
3. This marker binds to the constant region of IgG, so protein A or protein G fluorephore conjugates cannot be used as the secondary antibody.
|Monitoring protein migration during SDS-PAGE.
Verifying transfer efficiency on membranes for Western Blot.
Approximate sizing of proteins on SDS-PAGE gels.
Locating a protein of interest for excision from an unstained preparative gel.