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| M00575 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress Mus musculus adenosine A2A receptor (ADORA2A) on the surface and contain high levels of G protein Gαs to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 450 μg/ml Zeocin (Cat. No. R250-01, Life Technologies). |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma). |
Figure 1. Dose dependent stimulation of intracellular cAMP accumulation upon treatment with NECA in CHO-K1/mouse ADORA2A cells. d2 acceptor fluorophore -labeled cAMP (Cat. No. 62AM4PEC; Revvity) and intracellular cAMP in CHO-K1/mouse ADORA2A cells competitively bind with Europium Cryptate-labeled anti-cAMP monoclonal antibody. The FRET signal decreases as the intracellular cAMP concentration rises and was measured by plate reader (Pherastar, BMG). The EC50 of NECA on CHO-K1/mouse ADORA2A cells was 42.55 nM.
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
