Figure 1. h-CXCL12b/SDF-1β-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CXCR4 cells. The cells were loaded with Calcium-4 prior to stimulation with a CXCR4 receptor agonist h-CXCL12b/SDF-1β. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of h-CXCL12b/SDF-1β (Mean ± SD, n = 2). The EC50 of h-CXCL12b/SDF-1β on this cells was 1.05 µg/ml.
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom
CHO-K1/CXCR4/Gα15 Stable Cell Line