Figure 1. NDP-a-MSH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/MC4 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist NDP-a-MSH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NDP-a-MSH (Mean ± SD, n = 2). The EC₅₀ of NDP-a-MSH on this cell was 0.30 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC4/Gα15 Stable Cell Line

The melanocortin family consists of five melanocortin G-Protein-coupled receptors (designated MC1-5R). The melanocortins, α-, β- and γ-melanocyte-stimulating hormones (MSHs), are peptides derived from a precursor protein POMC, and play important roles in energy balance, reproductive function, pigmentation and inflammation. MC4 receptors with high affinities for α-MSH and ACTH are expressed almost exclusively in the CNS and appear to play a prominent role in energy homeostasis.

产品编号	M00272
规格	2 vials 定制报价
数量
价格	￥85000


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Synonyms

MC4 receptor, MC4R, melanocortin receptor 4, MC4-R

产品概述

Applications

Functional assay for MC4 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例

Figure 1. NDP-a-MSH-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/MC4 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist NDP-a-MSH. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of NDP-a-MSH (Mean ± SD, n = 2). The EC₅₀ of NDP-a-MSH on this cell was 0.30 μM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/MC4/Gα15 Stable Cell Line

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