High Affinity Ni-NTA Resin

Ni-NTA亲和层析介质（产品编号L00250）是把NTA（氮川三乙酸）共价偶联到4%琼脂糖介质上，再通过NTA的4个结合位点螯合Ni2+制备而成的亲和层析介质。Ni-NTA亲和层析介质的Ni2+脱落率较低，它能耐受蛋白纯化过程中使用的很多添加剂，并且有着较高的蛋白结合能力和稳定性，因此Ni-NTA亲和层析介质非常适用于多组氨酸重组蛋白的纯化。

价格	￥570
产品编号	L00250
规格	10 ml Resin (Total 20 ml) 10 ml Resin (with 5 columns) 25 ml Resin (Total 50 ml) 50 ml Resin (Total 100 ml) 100 ml Resin (Total 200 ml) 200 ml Resin (Total 400 ml) 大单询价
数量

产品概述产品属性应用指导图例

Description

Ni-NTA亲和层析介质（产品编号L00250）是把NTA（氮川三乙酸）共价偶联到4%琼脂糖介质上，再通过NTA的4个结合位点螯合Ni²⁺制备而成的亲和层析介质。Ni-NTA亲和层析介质的Ni²⁺脱落率较低，它能耐受蛋白纯化过程中使用的很多添加剂，并且有着较高的蛋白结合能力和稳定性，因此Ni-NTA亲和层析介质非常适用于多组氨酸重组蛋白的纯化。

产品概述

Key Features

产品特征

基质	4%琼脂糖
平均粒径	90 μm（45-165 μm）
吸附量	≥20 mg 6xHis-tagged 蛋白（27 kDa）
储存溶液	含20%乙醇的1×PBS

Ni-NTA亲和层析介质可耐受试剂

变性剂	表面活性剂	还原剂	盐	其他
6 M 盐酸胍	2%Triton X-100	20 mM β-ME	4 M MgCl₂	50%甘油
8 M尿素	2% Tween 20	1 mM DTT	5 mM CaCl₂	20%乙醇
——	1% CHAPS	——	2 M NaCl	1 mM EDTA

产品属性

Storage & Stability

2-8℃；DO NOT FREEZE

应用指导

Applications:

1. Purification of polyhistidine-tagged proteins under native conditions.
2. Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions.

图例

层析柱安装流程示意图 »

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High Affinity Ni-NTA Resin

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