| ￥2100 | |
| Z03708 | |
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| Product Introduction |
Benz-Neburase™ GMP, tag-free is a genetically
engineered endonuclease from Serratia marcescens that cleaves all forms of DNA
and RNA non-specifically under a broad range of conditions. Benz-Neburase™ Nuclease is mostly
utilized to remove residual DNA and RNA during production process of biological
molecules of interest in biopharmaceutical manufacturing including but not
limited to vaccine production, viral vector production, as well as in gene and
cell therapy-related manufacturing. |
| Expression System |
E.coli |
| Species |
Serratia marcescens |
| Tag |
Tag free |
| Theoretical Molecular Weight |
27.0 kDa |
| Biological Activity |
≥ 250.0 U/μl (or 1.1 × 106 U/mg) One unit is defined as the amount of enzyme that digests sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (within reaction volume 0.5 ml). |
| Storage Buffer |
20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% Glycerol, pH 8.0. |
| Storage & Stability | Store at -20±5 °C for up to 24 months or at 2-8 °C for 2 weeks from the date of manufacture. Avoid repeated freeze-thaw cycles. |
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Digests all forms of DNA and RNA (double stranded, single stranded,
linearized, and circular forms) under a board range of conditions. |
| Appearance |
Clear, colorless liquid |
| Purity |
≥ 99.0% as analyzed by SEC-HPLC ≥ 95.0% as analyzed by reducing SDS-PAGE |
| Enzyme Activity | ≥ 250.0 U/μl |
| Endotoxin Level |
< 0.01 EU/μg |
| Residual HCP |
≤ 20 ng/mg |
| Protease Activity |
Non-detectable |
| Bioburden |
< 1 CFU/ml |
| Mycoplasma |
Negative |
Figure 2: Benz-NeburaseTM Nuclease reduces viscosity of bacterial lysate by digesting nucleic acid. 2.5 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 1 ml of bacterial lysate. Incubate at 37 °C for 30 min and then centrifuge the samples and observe the viscosity of the precipitate and the supernatant. The test results show that Benz-Neburase™ GMP, tag free can greatly reduce the viscosity of bacterial lysate. »
Figure 3: Benz-NeburaseTM Nuclease prevents and reduces cell clumping. The adhered cells were spread in a 24-well plate and treated with control buffer (left) and 50 U/ml Benz-Neburase™ GMP, tag free (Cat. No. Z03708) (right) at 37 °C for 30 min. After the incubation, the cells were observed under a microscope. The test result show that Benz-Neburase™ GMP, tag free can efficiently reduce cell clumping. »
Figure 4: Benz-NeburaseTM Nuclease digests various types of nucleic acids. In a 20 μl reaction volume, 1 U of Benz-Neburase™ GMP, tag free (Z03708) was used to digest different kinds of nucleic acids (PCR product, genomic DNA, plasmid DNA, and RNA) at 37 °C for 10 min. The test results show that Benz-Neburase™ GMP, tag free is effective in digesting various forms of DNA and RNA. »
Figure 1: Benz-NeburaseTM Nuclease removes nucleic acid residues during AAV production. 100 U Benz-Neburase™ GMP, tag free (Cat. No. Z03708) was used to treat 2 ml of cell suspension at 37 °C for 60 min. Then the mix was centrifuged at 1600 g for 10 min to remove cells and cell debris, and measurements were taken for the HCD, plasmid DNA residues, and viral genome (Vg) recovery. The test results show that Benz-Neburase™ GMP, tag free can remove DNA and plasmid residue in AAV virus production process more effectively than the competing product. The usage of Benz-Neburase™ GMP, tag free has minimal impact on viral production. »
For laboratory research use only. Direct human use,
including taking orally and injection and clinical use are forbidden.
