THE™ c-Myc Tag Antibody, mAb, Mouse

THE™ c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells.It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.

价格	￥1500 在线订购包邮(港澳台除外)
产品编号	A00704-100
规格	100 μg 大单询价
数量
参考货期


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产品概述应用指导产品属性技术背景

产品概述

Specificity	THE™ c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells.It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.
Host Species	Mouse
Immunogen	A synthetic peptide EQKLISEEDL coupled to KLH
Conjugate	Unconjugated

应用指导

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.05-0.2 µg/ml
Western Blot	0.05-0.5 µg/ml
Western Blot	1:100-1:1,000
Immunoprecipitation (IP)	1 µg/ml
Immunocytochemistry/Immunofluorescence (ICC/IF)	1 µg/ml

产品属性

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a,κ
Clonality	Monoclonal
Clone ID	2G8D5

技术背景

Target Background

The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24.c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen.c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.

Synonyms

Mouse Anti-c-Myc-tag mAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

相关文献

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