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| A00704 | |
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| Specificity | THE™ c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells.It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide EQKLISEEDL coupled to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. | |||||||||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Storage Instructions | The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
| Purification | Protein A affinity column |
| Isotype | Mouse IgG2a,κ |
| Clonality | Monoclonal |
| Clone ID | 2G8D5 |
Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using
THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704) »
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG »
Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG »
20 μg Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704) and half of th IP was loaded on the gel.
The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232) »
Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.
Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 µg/ml) in assay.
The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG. »
| Target Background | The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24.c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen.c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays. |
| Synonyms | Mouse Anti-c-Myc-tag mAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
