THE™ His Tag Antibody, mAb, Mouse
| Synonyms | THETM Anti-His mAb; |
| Description | Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag. THETM His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody. THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins. THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Subclass | IgG1, k |
| Clone ID | 6G2A9 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | THETM His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components,etc. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western Blot: 0.1-0.2 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Flow cytometry (FACS): 1 µg/ml TR-FRET assays: user-optimized Other applications: user-optimized |
AL Farrow, et al. Immunization with Hexon Modified Adenoviral Vectors Integrated with gp83 Epitope Provides Protection against Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2014Aug.;8(8):e3089
Zhang Y, et al. The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the of human DNA polymerase α-primase complex. J Biol Chem. 2014June;
L Matthew, et al. Lys11- and Lys48-linked ubiquitin chains interact with p97 during endoplasmic-reticulum-associated degradation. Biochem J. 2014April;459(1):205-16
Duquesne S, et al. Construction of a Highly Active Xylanase Displaying Oleaginous Yeast: Comparison of Anchoring Systems. PLoS One. 2014April;9(4):
Kitevska T, et al. Analysis of the minimal specificity of caspase-2 and identification of Ac-VDTTD-AFC as a caspase-2-selective peptide substrate. Biosci Rep. 2014Feb.;
Wang Q.,et al. Enhancing catalytic activity of a hybrid xylanase through single substitution of Leu to Pro near the active site.World J Microbiol Biotechnol.2012Mar;28(3):929-935
Rubio-Infante N.,et al. A Chloroplast-Derived C4V3 Polypeptide From The Human Immunodeficiency Virus (Hiv) Is Orally Immunogenic In Mice.Plant Mol Biol.2012Mar;78(4-5):337-49
Jiang Qian.,et al. Interleukin-1R3 Mediates Interleukin-1¯Induced Potassium Current Increase Through Fast Activation Of Akt Kinase.Proc Natl Acad Sci U S A.2012Jul;109(30):12189 - 12194
Frank EG.,et al. A Strategy For The Expression Of Recombinant Proteins Traditionally Hard To Purify.Anal Biochem.2012Otc;429(2):132 - 9
Chen X.,et al. An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.Cell.2013Mar;153(1):166-77
West A.,et al. Npas4 Is Activated by Melatonin, and Drives the Clock Gene Cry1 in the Ovine Pars Tuberalis.Mol Endocrinol.2013Jun;27(6):979 - 989
Stenner F.,et al. RP1 Is a Phosphorylation Target of CK2 and Is Involved in Cell Adhesion.PLoS One.2013Jul;8(7):e67595
Son J.,et al. Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin.Genes Dev.2013Dec;27(24):2663-77
SS Khan.,et al. Delineation of Domains Within the Cannabinoid CB1 and Dopamine D2 Receptors That Mediate the Formation of the Heterodimer Complex.J Mol Neurosci.2013Nov;
Amparyup P.,et al. A Pattern Recognition Protein Binds To Lipopolysaccharide And Beta-1,3-Glucan And Activates The Shrimp Prophenoloxidase System.J Biol Chem.2012Mar;
请点击此处查找更多相关文献
Zhang Y, et al. The C-terminal domain of the DNA polymerase catalytic subunit regulates the primase and polymerase activities of the of human DNA polymerase α-primase complex. J Biol Chem. 2014June;
L Matthew, et al. Lys11- and Lys48-linked ubiquitin chains interact with p97 during endoplasmic-reticulum-associated degradation. Biochem J. 2014April;459(1):205-16
Duquesne S, et al. Construction of a Highly Active Xylanase Displaying Oleaginous Yeast: Comparison of Anchoring Systems. PLoS One. 2014April;9(4):
Kitevska T, et al. Analysis of the minimal specificity of caspase-2 and identification of Ac-VDTTD-AFC as a caspase-2-selective peptide substrate. Biosci Rep. 2014Feb.;
Wang Q.,et al. Enhancing catalytic activity of a hybrid xylanase through single substitution of Leu to Pro near the active site.World J Microbiol Biotechnol.2012Mar;28(3):929-935
Rubio-Infante N.,et al. A Chloroplast-Derived C4V3 Polypeptide From The Human Immunodeficiency Virus (Hiv) Is Orally Immunogenic In Mice.Plant Mol Biol.2012Mar;78(4-5):337-49
Jiang Qian.,et al. Interleukin-1R3 Mediates Interleukin-1¯Induced Potassium Current Increase Through Fast Activation Of Akt Kinase.Proc Natl Acad Sci U S A.2012Jul;109(30):12189 - 12194
Frank EG.,et al. A Strategy For The Expression Of Recombinant Proteins Traditionally Hard To Purify.Anal Biochem.2012Otc;429(2):132 - 9
Chen X.,et al. An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.Cell.2013Mar;153(1):166-77
West A.,et al. Npas4 Is Activated by Melatonin, and Drives the Clock Gene Cry1 in the Ovine Pars Tuberalis.Mol Endocrinol.2013Jun;27(6):979 - 989
Stenner F.,et al. RP1 Is a Phosphorylation Target of CK2 and Is Involved in Cell Adhesion.PLoS One.2013Jul;8(7):e67595
Son J.,et al. Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin.Genes Dev.2013Dec;27(24):2663-77
SS Khan.,et al. Delineation of Domains Within the Cannabinoid CB1 and Dopamine D2 Receptors That Mediate the Formation of the Heterodimer Complex.J Mol Neurosci.2013Nov;
Amparyup P.,et al. A Pattern Recognition Protein Binds To Lipopolysaccharide And Beta-1,3-Glucan And Activates The Shrimp Prophenoloxidase System.J Biol Chem.2012Mar;
请点击此处查找更多相关文献
| 产品编号: A00186 | ||
|---|---|---|
规格 |
价格 |
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| 100 ug | ¥2500 | |
