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资源 » 研究应用 » Stem Cell Research » c-Myc-tag Antibody, pAb, Rabbit

c-Myc-tag Antibody, pAb, Rabbit

Specificity This Antibody recognizes c-Myc tagged fusion proteins.
Host Species Rabbit
Immunogen c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunofluorescence 5-20 µg/ml
Immunoprecipitation (IP) 2-5 µg/ml
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Target Background The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24.c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen.c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins.This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Synonyms Rabbit Anti-c-Myc-tag pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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产品编号: A00172
规格
价格
282 A00172-40 40 μg ¥990