• 中文
  • 登录
  • 联系我们
×
目录产品 » 抗体产品 » THE金牌抗体 » 羊抗HA-tag多抗 » DYKDDDDK-tag Antibody, pAb, Rabbit

DYKDDDDK-tag Antibody, pAb, Rabbit

Specificity This Antibody recognizes DYKDDDDK tagged fusion proteins.
Host Species Rabbit
Immunogen A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Conjugate Unconjugated

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.05 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunofluorescence 5-20 µg/ml
Flow Cytometry 1-3 µg for 1 x 106 cells
Other applications User-optimized

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The antibody is stable in lyophilized form if stored at -20°C or below.The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Synonyms Rabbit Anti-DYKDDDDK-tag pAb;Rabbit Anti-Flag-tag pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Ji-Hee Kim, et al. Insulin-activated store-operated Ca entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. (2021-11)

Wang J, et al. Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex. Stem cell research & therapy. (2020-07)

Zehr EA, et al. Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules. Dev Cell. (2020)

ZhangHeng, et al. Correction: TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition. J. Biol. Chem. (2019-04)

LaFoya Bryce, et al. Src kinase phosphorylates Notch1 to inhibit MAML binding. Sci Rep. (2018)

Xiao B, et al. Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin. J Biol Chem. (2017-10)

Li Zhong, et al. Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction. Cell Res. (2017)

Kuga T, et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Scientific REPORTS. (2016-05)

McLaughlin D, et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. (2016-04)

Jason K. K. Low, et al. Protein substrates of the arginine methyltransferase Hmt1 identified by proteome arrays. Proteomics. (2016-02)

LiTingting, et al. Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal. J. Biol. Chem. (2014)

Q Gan, et al. Pax6 mediates ß-catenin signaling for self-renewal and neurogenesis by neocortical radial glial stem cells. Stem Cells. (2013-10)

Wei Huang, et al. The N-Terminal Phosphodegron Targets Taz/Wwtr1 Protein For ScfΒ-Trcp-Dependent Degradation In Response To Phosphatidylinositol 3-Kinase Inhibition. J Biol Chem. (2012-07)

Xin Zhou, et al. Targeted polyubiquitylation of RASSF1C by the Mule and SCFbeta-TrCP ligases in response to DNA damage. Biochem J. (2012-01)

Chen-Ying Liu, et al. PP1 Cooperates with ASPP2 to Dephosphorylate and Activate TAZ. J Biol Chem. (2011-02)

Chen-Ying Liu, et al. The Hippo Tumor Pathway Promotes TAZ Degradation by Phosphorylating a Phosphodegron and Recruiting the SCFβ-TrCP E3 Ligase. J Biol Chem. (2010-11)

Heng Zhang, et al. TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition. J Biol Chem. (2009-05)

Qunying Lei, et al. TAZ promotes cell proliferation and EMT and is inhibited by the Hippo pathway. Mol Cell Biol. (2008-04)

请点击此处查找更多相关文献




更多产品文件类型 >>

产品编号: A00170
规格
价格
278 A00170-40 40 μg ¥990