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DYKDDDDK-tag Antibody, pAb, Rabbit

This Antibody recognizes DYKDDDDK tagged fusion proteins.
¥990
A00170

Specificity This Antibody recognizes DYKDDDDK tagged fusion proteins.
Host Species Rabbit
Immunogen A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Conjugate Unconjugated
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Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.05 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunocytochemistry/Immunofluorescence (ICC/IF) 5-20 µg/ml
Flow Cytometry 1-3 µg for 1 x 106 cells
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Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
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DYKDDDDK-tag Antibody, PAb, Rabbit

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DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. »

DYKDDDDK-tag Antibody, PAb, Rabbit

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml) »

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 49 kD
Observed Size: 49 kD »

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD »

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Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Synonyms Rabbit Anti-DYKDDDDK-tag pAb;Rabbit Anti-Flag-tag pAb;
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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Feng-Lin Dong, et al. Exosome-derived circUPF2 enhances resistance to targeted therapy by redeploying ferroptosis sensitivity in hepatocellular carcinoma. J Nanobiotechnology. (2024-05)
Yang Xu, et al. Microfluidic Synthesis of Scalable Layer-by-Layer Multiple Antigen Nano-Delivery Platform for SARS-CoV-2 Vaccines. Vaccines (Basel). (2024-03)
Tian Xie , et al. Collaborative regulation of yeast SPT-Orm2 complex by phosphorylation and ceramide. Cell Rep . (2024-01)
Guang Shi, et al. Bend family proteins mark chromatin boundaries and synergistically promote early germ cell differentiation. Protein Cell. (2021-11)
Ji-Hee Kim, et al. Insulin-activated store-operated Ca entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. (2021-11)
Wang J, et al. Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex. Stem cell research & therapy. (2020-07)
Zehr EA, et al. Katanin Grips the β-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules. Dev Cell. (2020)
ZhangHeng, et al. Correction: TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition. J. Biol. Chem. (2019-04)
LaFoya Bryce, et al. Src kinase phosphorylates Notch1 to inhibit MAML binding. Sci Rep. (2018)
Xiao B, et al. Reactive oxygen species trigger Parkin/PINK1 pathway-dependent mitophagy by inducing mitochondrial recruitment of Parkin. J Biol Chem. (2017-10)
Li Zhong, et al. Existing drugs as broad-spectrum and potent inhibitors for Zika virus by targeting NS2B-NS3 interaction. Cell Res. (2017)
Kuga T, et al. FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes. Scientific REPORTS. (2016-05)
McLaughlin D, et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. (2016-04)
Jason K. K. Low, et al. Protein substrates of the arginine methyltransferase Hmt1 identified by proteome arrays. Proteomics. (2016-02)
LiTingting, et al. Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal. J. Biol. Chem. (2014)
Q Gan, et al. Pax6 mediates ß-catenin signaling for self-renewal and neurogenesis by neocortical radial glial stem cells. Stem Cells. (2013-10)
Wei Huang, et al. The N-Terminal Phosphodegron Targets Taz/Wwtr1 Protein For ScfΒ-Trcp-Dependent Degradation In Response To Phosphatidylinositol 3-Kinase Inhibition. J Biol Chem. (2012-07)
Xin Zhou, et al. Targeted polyubiquitylation of RASSF1C by the Mule and SCFbeta-TrCP ligases in response to DNA damage. Biochem J. (2012-01)
Chen-Ying Liu, et al. PP1 Cooperates with ASPP2 to Dephosphorylate and Activate TAZ. J Biol Chem. (2011-02)
Chen-Ying Liu, et al. The Hippo Tumor Pathway Promotes TAZ Degradation by Phosphorylating a Phosphodegron and Recruiting the SCFβ-TrCP E3 Ligase. J Biol Chem. (2010-11)
Heng Zhang, et al. TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition. J Biol Chem. (2009-05)
Qunying Lei, et al. TAZ promotes cell proliferation and EMT and is inhibited by the Hippo pathway. Mol Cell Biol. (2008-04)
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