| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The solution and solid phase chemical synthesis approaches are expensive and time-consuming given the multistep nature of the synthetic routes [13], [14], [15], [16], [17 … A DNA construct encoding the C terminus of the TM1788 gene was commercially synthesized by GenScript … | Get A Quote |
C-di-GMP has emerged as a prevalent bacterial messenger that controls a multitude of bacterial behaviors. Having access to milligram or gram quantities of c-di-GMP is essential for the biochemical and structural characterization of enzymes and effectors involved in c-di-GMP signaling. Although c-di-GMP can be synthesized using chemical methods， diguanylate cyclases (DGC)-based enzymatic synthesis is the most efficient method of preparing c-di-GMP today. Many DGCs are not suitable for c-di-GMP production because of poor protein stability and the presence of a c-di-GMP-binding inhibitory site (I-site) in most DGCs. We have identified and engineered a thermophilic DGC for efficient production of c-di-GMP for cha... More
