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Advances in affinity ligand-functionalized nanomaterials for biomagnetic separation.

Biotechnol. Bioeng.. 2016; 
FieldsConor,LiPeng,O'MahonyJames J,LeeG
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摘要

The downstream processing of proteins remains the most significant cost in protein production, and is largely attributed to rigorous chromatographic purification protocols, where the stringency of purity for biopharmaceutical products sometimes exceeds 99%. With an ever burgeoning biotechnology market, there is a constant demand for alternative purification methodologies, to ameliorate the dependence on chromatography, while still adhering to regulatory concerns over product purity and safety. In this article, we present an up-to-date view of bioseparation, with emphasis on magnetic separation and its potential application in the field. Additionally, we discuss the economic and performance benef... More

关键词

antibody fragments,bioseparation,micro-particles,nanoparticles,peptides,superparamagn