| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | Total RNA was isolated from tea leaves using a Quick RNA Isolation Kit (Huayueyang, Beijing, China), and 1μg of total RNA was reverse transcribed to generate first-strand cDNA using Reverse Transcriptase M-MLV (RNase H−) (TaKaRa, Dalian, China) according to the manufacturer’s instructions. To obtain the ORF of CsHSP17.2, a primer pair (ORF-F/-R, Table S2) was designed and employed for PCR amplification. The resulting amplicons were purified and cloned into the pEASY-T1 Simple Cloning Vector (Transgen, Beijing, China) for sequencing (Genscript, Nanjing, China). | Get A Quote |
Small heat shock proteins (sHSPs) play important roles in responses to heat stress. However, the functions of sHSPs in tea plants (Camellia sinensis) remain uncharacterized. A novel sHSP gene, designated CsHSP17.2, was isolated from tea plants. Subcellular localization analyses indicated that the CsHSP17.2 protein was present in the cytosol and the nucleus. CsHSP17.2 expression was significantly up-regulated by heat stress but was unaffected by low temperature. The CsHSP17.2 transcript levels increased following salt and polyethylene glycol 6000 treatments but decreased in the presence of abscisic acid. The molecular chaperone activity of CsHSP17.2 was demonstrated in vitro. Transgenic Escherichia coli and Pich... More
